rabbit polyclonal anti human  (Cell Signaling Technology Inc)

Journal: Experimental and Therapeutic Medicine

Article Title: Effects of negative-pressure wound therapy combinedwith microplasma on treating wounds of ulcer and the expression of heat shock protein 90

doi: 10.3892/etm.2017.4266

Figure Lengend Snippet: HSP90 protein expression of the wound tissues in each of the three groups was detected by western blotting. HSP90, heat shock protein 90.

Article Snippet: The membrane was blocked with 50 g/l skim milk for 1 h, the primary antibodies were polyclonal rabbit anti-human HSP90 antibody (dilution, 1:250; cat. no. BA1638) and rabbit polyclonal β-actin antibody (dilution, 1:400; cat. no. BA2305), and the secondary antibodies were goat anti-rabbit antibody (dilution, 1:5,000; cat. no. BA1039) (all from Wuhan Boster Biological Technology, Ltd., Wuhan, China) labeled by horseradish peroxidase.

Techniques: Expressing, Western Blot

Journal: Scientific Reports

Article Title: Activation of EGFR by small compounds through coupling the generation of hydrogen peroxide to stable dimerization of Cu/Zn SOD1

doi: 10.1038/srep21088

Figure Lengend Snippet: ( a ) MDA MB468 cells were transfected with SOD1 siRNA (SOD1 siRNA) or not transfected (No siRNA), and exposed to 0.4% DMSO or 100 μM NSC 228155 or CN 009543V for 10 min. Cells extracts were analyzed by western blotting. Shown are results from a representative experiment. ( b ) The signal intensity of protein bands was quantified with C-Digit software (Li-COR). The relative rate of EGFR phosphorylation was normalized to the loading control Hsp90α, and the value obtained was compared with that of cells exposed to a vehicle (equal to 1 on the histograms). The relative abundance of monomeric and dimeric forms of SOD1 was quantified as the ratio of corresponding bands (%) in non-transfected cells.

Article Snippet: Human protein EGF, goat anti-human EGFR polyclonal antibody conjugated to biotin, mouse anti-human pEGFR Tyr1068 and anti-human Cu/Zn SOD1 mAbs, and rabbit anti-Hsp90α polyclonal antibody (loading control of cytoplasmic proteins) were from R&D Systems.

Techniques: Transfection, Western Blot, Software, Relative Rate

Journal: Experimental and Therapeutic Medicine

Article Title: Effect of a heat shock protein 90-specific inhibitor on the proliferation and apoptosis induced by VEGF-C in cervical cancer cells

doi: 10.3892/etm.2014.1930

Figure Lengend Snippet: Western blot analysis results of Hsp90 protein expression bands induced by VEGF-C at different times in HeLa cells. Lane 1: Control; Lane 2: VEGF-C (50 ng/μl) 3 h; Lane 3: VEGF-C (50 ng/μl) 6 h; Lane 4: VEGF-C (50 ng/μl) 12 h; Lane 5: VEGF-C (50 ng/μl) 24 h. Hsp90, heat shock protein 90; VEGF-C, vascular endothelial growth factor-C.

Article Snippet: GA was purchased from Alexis Biochemicals (San Diego, CA, USA) and Hsp90 rabbit anti-human polyclonal antibody was obtained from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Techniques: Western Blot, Expressing, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Effect of a heat shock protein 90-specific inhibitor on the proliferation and apoptosis induced by VEGF-C in cervical cancer cells

doi: 10.3892/etm.2014.1930

Figure Lengend Snippet: Western blot analysis results of Hsp90 protein expression bands induced by VEGF-C in the presence or absence of various inhibitors in HeLa cells. Lane 1: Control; Lane 2: VEGF-C + KDR-Ab; Lane 3: VEGF-C + PD98059; Lane 4: VEGF-C + LY294002; Lane 5: VEGF-C + GA. Hsp90, heat shock protein 90; GA, geldanamycin; VEGF-C, vascular endothelial growth factor-C.

Article Snippet: GA was purchased from Alexis Biochemicals (San Diego, CA, USA) and Hsp90 rabbit anti-human polyclonal antibody was obtained from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Techniques: Western Blot, Expressing, Control